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Anna Katharina Hildebrandt

The t(8;21) chromosomal translocation activates aberrant expression of the AML1-ETO (AE) fusion protein and is commonly associated with core binding factor acute myeloid leukaemia (CBF AML). Combining a conditional mouse model that closely resembles the slow evolution and the mosaic AE expression pattern of human t(8;21) CBF AML with global transcriptome sequencing, we find that disease progression was characterized by two principal pathogenic mechanisms.
Mass spectrometry is the major analytical tool for the identification and quantification of proteins in biological samples. In so-called top-down proteomics, separation and mass spectrometric analysis is performed at the level of intact proteins, without preparatory digestion steps. It has been shown that the tandem mass tag (TMT) labeling technology, which is often used for quantification based on digested proteins (bottom-up studies), can be applied in top-down proteomics as well.
Structural biology is based on an important observation: the function of a biomolecule is determined by its three-dimensional structure and its physico-chemical properties. Hence, visualization, modeling, and simulation of molecular structures and of their properties are crucial tools of the field. Typically, the graphical interfaces to molecular modeling packages are aimed at domain experts with significant experience and require an extensive learning period.

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